Product Details
FireProt 2.0
Created: 2023
Ježík Andrej, Bc. (FIT BUT)
Horáčková Jana, Mgr. (LL)
Borko Simeon, Ing. (FIT BUT)
Kabourek Petr (LL)
Damborský Jiří, prof. Mgr., Dr. (SCI MUNI)
Bednář David, Mgr. (LL)
protein engineering, thermostable proteins, multiple-point mutants, protein stability
FireProt protocol enables the automated computational design of highly stable multiple-point mutants. FireProt 2.0 builds on top of the previously published FireProt web, retaining the original functionality and expanding it with several new stabilization strategies. FireProt 2.0 integrates the AlphaFold database and the homology modelling for structure prediction, enabling calculations starting from a sequence. Multiple-point designs are constructed using the Bron-Kerbosch algorithm minimizing the antagonistic effect between the individual mutations. Users can newly limit the FireProt calculation to a set of user-defined mutations, run a saturation mutagenesis of the whole protein, or select rigidifying mutations based on B-factors. Evolution-based back-to-consensus strategy is complemented by ancestral sequence reconstruction. FireProt 2.0 is significantly faster and a reworked graphical user interface broadens the tool's availability even to users with older hardware.