Publication Details

Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization

DVOŘÁK Pavel, KURUMBANG Nagendra, BENDL Jaroslav, BREZOVSKÝ Jan, PROKOP Zbyněk and DAMBORSKÝ Jiří. Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. ChemBioChem, vol. 15, no. 13, 2014, pp. 1891-1895. ISSN 1439-4227. Available from: http://onlinelibrary.wiley.com/doi/10.1002/cbic.201402265/full
Czech title
Maximalizace efektivity multi-enzymového procesu pomocí optimalizace stechiometrie
Type
journal article
Language
english
Authors
Dvořák Pavel, Mgr. (LL)
Kurumbang Nagendra, Ph.D. (LL)
Bendl Jaroslav, Ing., Ph.D. (FIT BUT)
Brezovský Jan, Mgr., Ph.D. (LL)
Prokop Zbyněk, doc. RnDr., Ph.D. (LL)
Damborský Jiří, prof. Mgr., Dr. (LL)
URL
Keywords

biocatalysis, biotransformation, kinetic modelling

Abstract

Multi-enzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of biocatalysts’ stoichiometry. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalysing a five-step chemical conversion. Kinetic models of pathways featuring either wild-type or engineered enzymes were built and the enzyme stoichiometry of each pathway was optimized. Mathematical modelling and one-pot multi-enzyme laboratory experiments provided detailed insights into pathway dynamics, enabled the selection of suitable engineered enzyme and afforded high efficiency while minimizing biocatalyst loadings. The optimizing of stoichiometry in a pathway with engineered enzyme reduced the total biocatalyst load by an impressive 56 %. Our new workflow represents a broadly applicable strategy for optimizing multi-enzyme processes.

Published
2014
Pages
1891-1895
Journal
ChemBioChem, vol. 15, no. 13, ISSN 1439-4227
Publisher
WILEY
DOI
10.1002/cbic.201402265
UT WoS
000341586100007
BibTeX 
@ARTICLE{FITPUB10595,
   author = "Pavel Dvo\v{r}\'{a}k and Nagendra Kurumbang and Jaroslav Bendl and Jan Brezovsk\'{y} and Zbyn\v{e}k Prokop and Ji\v{r}\'{i} Damborsk\'{y}",
   title = "Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization",
   pages = "1891--1895",
   journal = "ChemBioChem",
   volume = 15,
   number = 13,
   year = 2014,
   ISSN = "1439-4227",
   doi = "10.1002/cbic.201402265",
   language = "english",
   url = "https://www.fit.vut.cz/research/publication/10595"
}
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