Publication Details

Fully Automated Ancestral Sequence Reconstruction using FireProtASR

KHAN Rayyan T., MUSIL Miloš, ŠTOURAČ Jan, DAMBORSKÝ Jiří and BEDNÁŘ David. Fully Automated Ancestral Sequence Reconstruction using FireProtASR. Current Protocols, vol. 1, no. 2, 2021, pp. 1-5. ISSN 1934-3396. Available from: https://currentprotocols.onlinelibrary.wiley.com/doi/full/10.1002/cpz1.30
Czech title
Plně automatizovaná ancestrální rekonstrukce s využitím FireProtASR
Type
journal article
Language
english
Authors
Khan Rayyan T., M.Sc. (LL)
Musil Miloš, Ing., Ph.D. (DIFS FIT BUT)
Štourač Jan (LL)
Damborský Jiří, prof. Mgr., Dr. (LL)
Bednář David, Mgr. (LL)
URL
Keywords

ancestral reconstrution, stable proteins, protein engineering, specificity

Abstract

Protein evolution and protein engineering techniques are of great interest in basic science and industrial applications such as pharmacology, medicine, or biotechnology. Ancestral sequence reconstruction (ASR) is a powerful technique for probing evolutionary relationships and engineering robust proteins with good thermostability and broad substrate specificity. The following protocol describes the setting up and execution of an automated FireProtASR workflow using a dedicated web site. The service allows for inference of ancestral proteins automatically, from a single protein sequence. Once a protein sequence is submitted, the server will build a dataset of homology sequences, perform a multiple sequence alignment (MSA), build a phylogenetic tree, and reconstruct ancestral nodes. The protocol is also highly flexible and allows for multiple forms of input, advanced settings, and the ability to start jobs from: (i) a single sequence, (ii) a set of homologous sequences, (iii) an MSA, and (iv) a phylogenetic tree. This approach automates all necessary steps and offers a way for novices with limited exposure to ASR techniques to improve the properties of a protein of interest. The technique can even be used to introduce catalytic promiscuity into an enzyme. A web server for accessing the fully automated workflow is freely accessible at https://loschmidt.chemi.muni.cz/fireprotasr/

Published
2021
Pages
1-5
Journal
Current Protocols, vol. 1, no. 2, ISSN 1934-3396
Publisher
WILEY
DOI
10.1002/cpz1.30
EID Scopus
2-s2.0-85102178516
BibTeX 
@ARTICLE{FITPUB12273,
   author = "T. Rayyan Khan and Milo\v{s} Musil and Jan \v{S}toura\v{c} and Ji\v{r}\'{i} Damborsk\'{y} and David Bedn\'{a}\v{r}",
   title = "Fully Automated Ancestral Sequence Reconstruction using FireProtASR",
   pages = "1--5",
   journal = "Current Protocols",
   volume = 1,
   number = 2,
   year = 2021,
   ISSN = "1934-3396",
   doi = "10.1002/cpz1.30",
   language = "english",
   url = "https://www.fit.vut.cz/research/publication/12273"
}
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