Publication Details
Comparison of Fungal Thermophilic and Mesophilic Catalase-Peroxidases for Their Antioxidative Properties
Musil Miloš, Ing., Ph.D. (DIFS FIT BUT)
Bednář David, Mgr. (LL)
Chovanová Katarína (SAV)
Bauerová-hlinková Vladena (SAV)
Bellová Jana (SAV)
Kohútová Lenka (SAV)
Baráth Peter (SAV)
Zámocký Marcel (SAV)
peroxidase-catalase superfamily, heme catalase, bifunctional enzyme, reactive oxygen species, oxidative stress
Catalase-peroxidases (KatGs) are unique bifunctional oxidoreductases that contain heme in their active centers allowing both the peroxidatic and catalatic reaction modes. These originally bacterial enzymes are broadly distributed among various fungi allowing them to cope with reactive oxygen species present in the environment or inside the cells. We used various biophysical, biochemical, and bioinformatics methods to investigate differences between catalase-peroxidases originating in thermophilic and mesophilic fungi from different habitats. Our results indicate that the architecture of the active center with a specific post-translational modification is highly similar in mesophilic and thermophilic KatG and also the peroxidatic acitivity with ABTS, guaiacol, and L-DOPA. However, only the thermophilic variant CthedisKatG reveals increased manganese peroxidase activity at elevated temperatures. The catalatic activity releasing molecular oxygen is comparable between CthedisKatG and mesophilic MagKatG1 over a broad temperature range. Two constructed point mutations in the active center were performed selectively blocking the formation of described post-translational modification in the active center. They exhibited a total loss of catalatic activity and changes in the peroxidatic activity. Our results indicate the capacity of bifunctional heme enzymes in the variable reactivity for potential biotech applications.
@ARTICLE{FITPUB13061,
author = "Andrej Poljovka and Milo\v{s} Musil and David Bedn\'{a}\v{r} and Katar\'{i}na Chovanov\'{a} and Vladena Bauerov\'{a}-hlinkov\'{a} and Jana Bellov\'{a} and Lenka Koh\'{u}tov\'{a} and Peter Bar\'{a}th and Marcel Z\'{a}mock\'{y}",
title = "Comparison of Fungal Thermophilic and Mesophilic Catalase-Peroxidases for Their Antioxidative Properties",
pages = "1--19",
journal = "Antioxidants",
volume = 12,
number = 7,
year = 2023,
ISSN = "2076-3921",
doi = "10.3390/antiox12071382",
language = "english",
url = "https://www.fit.vut.cz/research/publication/13061"
}